To evaluate the anti-aging action of NMN in vitro, scientists assessed the effects of NMN on cellular mtROS production and mitochondrial phenotype in cultured primary cerebromicrovascular endothelial cells (CMVECs). NMN attenuates mitochondrial oxidative stress and improves mitochondrial bioenergetics in aged cerebromicrovascular endothelial cells. NMN treatment restores NO release in aged CMVECs in vitro and that NMN supplementation also rescues endothelial NO-mediated vasodilation in the aortas of aged mice[1].